Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture
Biocell
; 33(3): 179-186, Dec. 2009. ilus, tab
Article
in English
| BINACIS
| ID: bin-127221
Responsible library:
AR40.1
ABSTRACT
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 microM thidiazuron and 4.44 microM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 microM alpha-naphthalenacetic acid, 13.95 microM kinetin and 13.32 microM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 microM alpha-naphthalenacetic acid.(AU)
Full text:
Available
Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Plant Growth Regulators
/
Cells, Cultured
/
Organogenesis
/
Fabaceae
Language:
English
Journal:
Biocell
Year:
2009
Document type:
Article
Institution/Affiliation country:
Universidad Nacional del Nordeste/Argentina