Autophagy, apoptosis and organelle features during cell exposure to cadmium
Biocell
; 37(2): 45-54, ago. 2013. ilus, graf
Article
in English
| BINACIS
| ID: bin-130398
Localization: AR40.1
ABSTRACT
Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 microM - 20 microM) and exposure times (2 h - 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 microM for 12 h led to irreversible damages. Before these drastic and irreparable damages, treated cells (5 microM for 12 h) presented a progressive loss of mitochond rial function and cytoplasm acidification as well as dysfunction and disorganization of microfilaments and endoplasmic reticulum. These damages led to the induction of apoptotic events and an increase in autophagic bodies in the cytoplasm. These results revealed that Cd affects multiple intra-cellular targets that induce alterations in the mitochondria, cytoskeleton, endoplasmic reticulum and acidic compartments, ultimately culminating in cell death via apoptotic and autophagic pathways.(AU)
Full text:
Available
Collection:
National databases
/
Argentina
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Liver Cancer
Database:
BINACIS
Main subject:
Autophagy
/
Cadmium
/
Organelles
/
Apoptosis
/
Liver
Limits:
Humans
Language:
English
Journal:
Biocell
Year:
2013
Document type:
Article
Institution/Affiliation country:
Universidade Estadual do Norte Fluminense/Brasil