Effects of methylene blue on the development of intrinsic contractile responses of the guinea pig tracheal smooth muscle
Acta physiol. pharmacol. ther. latinoam
; 47(3): 157-64, 1997. ilus, gra
Article
in English
| BINACIS
| ID: bin-20670
Responsible library:
BR1.1
ABSTRACT
The aim of the present study was to investigate the effects of methylene bue, a guanylyl cyclase inhibitor, on the development of intrinsic contractile responses of the guinea pig tracheal smooth muscle. Paired tracheal chains were mounted for isotonic contractions under 500 mg of tension in Krebs-Henseleit solution. The intrinsic contractile tone modulated carbachol-induced contractions and was inhibited by indomethacin, suggesting the involvement of cyclooxygenase products on this tone. Methylene blue (5x10(-5)M) irreversibly inhibited the intrinsic contractile responses. considering that methylene blue prevents any endogenous production of cGMP, it would be expected to enhance the contractions. However, since methylene blue has effects over nitric oxide synthase and nitric oxide itself, we suggest that guanylyl cyclase activations is not important for the development of the intrinsic tone. (AU)
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Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Enzyme Inhibitors
/
Guanylate Cyclase
/
Methylene Blue
/
Muscle Contraction
/
Muscle, Smooth
Limits:
Animals
Language:
English
Journal:
Acta physiol. pharmacol. ther. latinoam
Year:
1997
Document type:
Article