Non enzymatic liver cells isolation: citrate-perchlorate technique
Acta physiol. pharmacol. ther. latinoam
; 46(2): 144-8, 1996. ilus
Article
in English
| BINACIS
| ID: bin-22352
Responsible library:
BR1.1
ABSTRACT
In the present study we describe a non-enzymatic technique for the isolation of rat hepatocytes by perfusion of liver through portal vein. The perfusion media consist of 1 mM sodium percholorate. 5 mM sodium citrate, 10 mM glucose, 129 mM NaCl and 0.1 per cent bovine-serum albumin at pH 7.4. After purification through diatrizoate gradient, electron microscopical studies revealed that most of purified hepatocytes were well preserved and presented a normal ultrastructure, thus correlating with previous biochemical results. The present method enables the recovery of metabolically and morphologically normal hepatocytes. (AU)
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Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Cell Separation
/
Citrates
/
Sodium Compounds
/
Perchlorates
/
Liver
Limits:
Animals
Language:
English
Journal:
Acta physiol. pharmacol. ther. latinoam
Year:
1996
Document type:
Article