DNA synthesis and nucleolar organizer regions circadian rhythm in mouse regenerating liver hepatocytes.
Biocell
; Biocell;24(2): 151-5, 2000 Aug.
Article
in En
| BINACIS
| ID: bin-39798
Responsible library:
AR1.1
ABSTRACT
DNA synthesis and Nucleolar Organizer Regions (NORs) were studied in C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) incorporated into DNA with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections of regenerating liver. Tissue samples were obtained at different times after hepatectomy along a circadian span. The results showed a strong correlation of values between DNA synthesis (BrdU labelling index) and AgNOR numbers, with higher counts during the activity period of animals at 0000/38 and 0400/42 hours Time of Day/Hours Post-Hepatectomy (TD/HPH), being the differences with other time points highly significant. Our observations demonstrate the existence of a strong correlation of DNA synthesis measured by BrdU incorporation and AgNOR numbers with a defined circadian rhythm in mouse regenerating hepatocytes.
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Collection:
06-national
/
AR
Database:
BINACIS
Language:
En
Journal:
Biocell
Year:
2000
Document type:
Article
Country of publication:
Argentina