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Comparative analysis of three methods to assess viability of mammalian cells in culture.
da Costa, A O; de Assis, M C; Marques, E de A; Plotkowski, M C.
Affiliation
  • Plotkowski, M C; Department of Microbiology and Immunology, State University of Rio de Janeiro UERJ, Brazil..
Biocell ; 23(1): 65-72, 1999 Apr.
Article in English | BINACIS | ID: bin-40091
Responsible library: AR1.1
ABSTRACT
Release of lactate dehydrogenase (LDH) from the cytoplasmic compartment, trypan blue exclusion and methylthiazole tetrazolium (MTT) colorimetric assays were compared with regard to their sensitivity in detecting damage of human cultured epithelial cells induced by sodium fluoride or puromycin. LDH assay did not detect any difference between controls and cells treated with either of the two drugs. Cell monolayers treated with 0.3
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Collection: National databases / Argentina Database: BINACIS Language: English Journal: Biocell Year: 1999 Document type: Article
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Collection: National databases / Argentina Database: BINACIS Language: English Journal: Biocell Year: 1999 Document type: Article