Estudio comparativo de métodos microbianos para la detección de tóxicos genéticos. / [Comparative study of microbial methods for the detection of genetic toxicity]

ABSTRACT

It is important to have a rapid and accurate method to detect the toxic action of drugs and chemical compounds used by man. A comparative study with two microbial systems was carried out one using Salmonella typhimurium and the other using Bacillus subtilis. The 1-8-dihydroxyantraquinone was the tested drug and the N-methyl-N-nitro-N-nitrosoguanidine and the 2-aminofluorene were used as control substances. These compounds were used as such or after previous transformation by a microsomal system. The results obtained showed that both systems the S. typhimurium and the B. subtilis work in a similar way, but the latter allows a more direct action of drug on the genetic material. The effect of the solvents ethanol and dimethylsulfoxide was analysed, because they affected the transformation and reversion processes that were carried out.