Empleo de epimastigotes formolados para la detección de anticuerpos anti-Trypanosoma cruzi mediante técnicas inmunoenzimáticas. / [Use of formalinized epimastigotes for the detection of anti-Trypanosoma cruzi antibodies using immunoenzyme technics]

ABSTRACT

Formalin-fixed epimastigotes of T. cruzi were employed to develop an ELISA and a Dot-immunobinding assay (Dot-IA). The results were compared with those obtained by the indirect fluorescent antibody test (IFA). Fifty positive and twenty negative sera for Chagas disease, supplied by the reference center (Institute Mario Fatala Chaben, Buenos Aires, Argentina), were analyzed. When the quantitative indirect ELISA was performed, no overlapping between positive and negative sera was observed and the correlation with IFA was 100