Trypanosoma cruzi: conditions required to improve metacyclic differentiation in axenic culture.
Rev. argent. microbiol
; 21(1): 9-14, 1989 Jan-Mar.
Article
in English
| BINACIS
| ID: bin-52020
Responsible library:
AR32.1
ABSTRACT
This work is a study on the behaviour of Epi from various T. cruzi strains and clones grown in media stimulating differentiation such as M 16, TAUP, TAUS, LIT-hemin and G-IH. Results showed that in our experimental conditions the G-IH medium was the only suitable one to induce Epi-Mtc morphogenesis. Optimal temperature for both multiplication and differentiation in this medium was 28 degrees C. The use of G-IH medium after nutrient depletion by dilution failed to induce differentiation. Parasites obtained from biphasic medium at 24-48 h culture proved the best to achieve morphogenesis. External Ca++ supply blockage affected the differentiation capacity of Tul and RA strains to a variable degree.
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Collection:
National databases
/
Argentina
Health context:
Neglected Diseases
Health problem:
Chagas Disease
/
Neglected Diseases
Database:
BINACIS
Language:
English
Journal:
Rev. argent. microbiol
Year:
1989
Document type:
Article