Formación y regeneración de protoplastos de una cepa nativa de Clostridium acetobutylicum. / [Formation and regeneration of protoplasts of a native strain of Clostridium acetobutylicum]

ABSTRACT

A procedure was optimized for the formation and regeneration of protoplasts in a Clostridium acetobutylicum strain isolated from cassava roots in Misiones, Argentina. The regeneration frequency was calculated as the ratio of regenerants (colony forming units on regeneration plates minus colony forming units from osmotically resistant cells) per initial cell number (colony forming units before protoplast formation). The percentage regeneration varied from 10 to 15