Nuevos mecanismos involucrados en la patogenesis de adenomas hipofisarios / New mechanisms involved in the pathogenesis of pituiatary adenomas
Medicina [B.Aires]
; 63(2): 147-150, 2003. tab, graf
Article
in Spanish
| BINACIS
| ID: bin-6095
Responsible library:
BR1.1
ABSTRACT
We studied Smad-4dn tumors generated from lactosomatotrophic GH3 cells stably transfected with a dominant negative form of Smad-4 (a bone morphogenetic protein-4, BMP-4, signal co-transducer) which had reduced tumorigenicity in nude mice, but had showed a late increase in tumor size. We found that they had lost in vivo the expression of Smad-4dn and had recovered c-Myc expression. In accordance, BMP-4 is overexpressed and stimulates the expression of c-Myc in human prolactinomas, but not in other human pituitary adenomas or normal pituitary. In addition ICI 182,780 inhibited BMP-4 stimulated c-Myc expression and BMP-4 and 17 beta-estradiol in combination had an additive effect on GH3 cell proliferation. Their action was inhibited by blocking BMP-4 with ICI 182,780 or 17 beta-estradiol with Smad-4dn. Furthermore, co-immunoprecipitation studies demonstrate that Smad-4 physically interacts with the ER alpha/ER beta. We show for the first time the role of BMP-4 in prolactinoma pathogenesis, involving a functional cross-talk BMP-4/E2 (AU)
Full text:
Available
Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Pituitary Neoplasms
/
Prolactinoma
/
Bone Morphogenetic Proteins
Type of study:
Etiology study
Limits:
Animals
/
Humans
Language:
Spanish
Journal:
Medicina [B.Aires]
Year:
2003
Document type:
Article
/
Congress and conference
Institution/Affiliation country:
Consejo Nacional de Investigaciones Científicas y Técnicas/Argentina
/
Hospital Santa Lucía/Argentina
/
Universidad de Buenos Aires/Argentina