Serotonin reverses the inhibitory effect of a brain soluble fraction on synaptosomal membrane Na+, K(+)-ATPase activity
Biocell
; 19(2): 153-157, Aug. 1995.
Article
in English
| BINACIS
| ID: bin-6335
Responsible library:
BR1.1
ABSTRACT
Normal operation of the Na+ pump (Na+, K(+)-ATPase) is essential for the maintenance of neurotransmission. Filtration through Sephadex G-50 of a brain soluble fraction allowed the separation of peaks I and II fractions, respectively stimulating and inhibiting synaptosomal membrane Na+, K(+)-ATPase activity. Peaks I and II were isolated from rat cerebral cortex and their effect together with serotonin (5-HT) was studied on ATPase activity by estimating K(+)-p-nitrophenylphosphatase activity in brain cortex synaptosomal membranes. It was observed that 10(-5) or 10(-4) M 5-HT failed to modify either control membrane enzyme activity or peak I activatory effect; on the other hand, such 5-HT concentrations significantly suppressed peak II inhibitory effect. This ability of 5-HT to reverse the inhibitory effect of endogenous factors on Na+, K(+)-ATPase activity could well be a new 5-HT modulatory action within the brain.(AU)
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Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Synaptosomes
/
Serotonin
/
Sodium-Potassium-Exchanging ATPase
Limits:
Animals
Language:
English
Journal:
Biocell
Year:
1995
Document type:
Article
Institution/Affiliation country:
Universidad de Buenos Aires/Argentina