Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
Biocell
; 24(3): 217-222, Dec. 2000.
Article
in English
| BINACIS
| ID: bin-6422
Responsible library:
BR1.1
ABSTRACT
The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility.(AU)
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Collection:
National databases
/
Argentina
Database:
BINACIS
Main subject:
Trypanosoma cruzi
/
Signal Transduction
/
Cell Movement
/
Nitric Oxide Synthase
/
Nitric Oxide
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Biocell
Year:
2000
Document type:
Article
Institution/Affiliation country:
Universidad de Buenos Aires/Argentina