Nuclear export of replication protein A in the non-replicative infective forms of Trypanosoma cruzi
FEBS Lett, fev. 2020
Article
in English
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: bud-2945
Responsible library:
BR78.1
ABSTRACT
Replication Protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA-binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a Nuclear Export Signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms.
Full text:
Available
Collection:
National databases
/
Brazil
Health context:
Neglected Diseases
Health problem:
Chagas Disease
/
Neglected Diseases
Database:
Sec. Est. Saúde SP
/
SESSP-IBPROD
Language:
English
Journal:
FEBS Lett
Year:
2020
Document type:
Article