Cultivo de las celulas estromales de la mèdula òsea en un medio suplementado con los nutrientes N2: implicaciones sobre la generaciòn de cèlulas neurales / s. t
Rev Neurol
; 40(2)Jan. 2005.
Article
in Spanish
| CUMED
| ID: cum-40075
Responsible library:
CU1.1
ABSTRACT
Most of the culture system for in vitro maintenance and neural differentiation of marrow stromal cells (MSCs) use synthetic media supplemented with 10 or 20 percent fetal bovine serum (FBS). Serum, however, is comprised of unknown quantities of undefined substances which could interfere the effect of exogenous substances on neural differentiation of MSCs, AIM. Here we describe survival of MSCs cultured in culture conditions where serum was reduced at 0,5 and 1 percent using Bottenstein and Sato's N2 formula (1979) and poly-L-lysine (PLL)-coated substrate...(AU)
Full text:
Available
Collection:
National databases
/
Cuba
Database:
CUMED
Main subject:
Bone Marrow Cells
/
Stromal Cells
/
Cell Culture Techniques
/
Culture Media
Limits:
Animals
Language:
Spanish
Journal:
Rev Neurol
Year:
2005
Document type:
Article
Institution/Affiliation country:
Centro Internacional de Restauración Neurológica/Cuba