A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomy cescerevisiae and Saccharomyces pastorianus
Int. microbiol
; 14(4): 201-206, dic. 2011. ilus, tab
Article
in En
| IBECS
| ID: ibc-102984
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species (AU)
RESUMEN
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Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Saccharomyces
/
Saccharomyces cerevisiae
/
Genetic Vectors
Language:
En
Journal:
Int. microbiol
Year:
2011
Document type:
Article