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Essential role of the czc determinant for cadmium, cobalt and zinc resistance in Gluconacetobacter diazotrophicus PAl 5
Intorne, Aline C; Oliveira, Marcos Vinicius V de; Pereira, Leandro de M; Souza Filho, Gonçalo A de.
Affiliation
  • Intorne, Aline C; State University of North Fluminense Darcy Ribeiro (UENF). Laboratory of Biotechnology. Rio de Janeiro. Brazil
  • Oliveira, Marcos Vinicius V de; State University of North Fluminense Darcy Ribeiro (UENF). Laboratory of Biotechnology. Rio de Janeiro. Brazil
  • Pereira, Leandro de M; Oswaldo Cruz Foundation (FIOCRUZ). Laboratory of Computational Biology and Systems. Rio de Janeiro. Brazil
  • Souza Filho, Gonçalo A de; State University of North Fluminense Darcy Ribeiro (UENF). Laboratory of Biotechnology. Rio de Janeiro. Brazil
Int. microbiol ; 15(2): 69-78, jun. 2012. ilus, tab
Article in English | IBECS | ID: ibc-102995
Responsible library: ES1.1
Localization: BNCS
ABSTRACT
The mechanisms of cadmium, cobalt and zinc resistance were characterized in the plant-growth-promoting bacterium Gluconacetobacter diazotrophicus PAl 5. The resistance level of the wild-type strain was evaluated through the establishment of minimum inhibitory concentrations (MIC) of the soluble compounds CdCl2·H2O, CoCl2·6H2O and ZnCl2. Gluconacetobacter diazotrophicus PAl 5 was resistant to high concentrations of Cd, Co and Zn, with MICs of 1.2, 20 and 20 mM, respectively. Screening of an insertion library from transposon EZ-Tn5 in the presence of ZnO revealed that the mutant GDP30H3 was unable to grow in the presence of the compound. This mutant was also highly sensitive to CdCl2·H2O, CoCl26H2O and ZnCl2. Molecular characterization established that the mutation affected the czcA gene, which encodes a protein involved in metal efflux. In silico analysis showed that czcA is a component of the czcCBARS operon together with four other genes. This work provides evidence of the high tolerance of G. diazotrophicus PAl 5 to heavy metals and that czc is a determinant for metal resistance in this bacterium (AU)
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Collection: National databases / Spain Database: IBECS Main subject: Metals, Heavy / Gluconacetobacter Language: English Journal: Int. microbiol Year: 2012 Document type: Article Institution/Affiliation country: Oswaldo Cruz Foundation (FIOCRUZ)/Brazil / State University of North Fluminense Darcy Ribeiro (UENF)/Brazil
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Collection: National databases / Spain Database: IBECS Main subject: Metals, Heavy / Gluconacetobacter Language: English Journal: Int. microbiol Year: 2012 Document type: Article Institution/Affiliation country: Oswaldo Cruz Foundation (FIOCRUZ)/Brazil / State University of North Fluminense Darcy Ribeiro (UENF)/Brazil