Dark fermentation: isolation and characterization of hydrogen-producing strains from sludges
Int. microbiol
; 16(1): 53-62, mar. 2013. ilus, tab
Article
in English
| IBECS
| ID: ibc-114745
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
To improve bacterial hydrogen production, ten hydrogen-producing strains belonging to Clostridium spp. were isolated from various sludges under low vacuum. Hydrogenogenesis by dark fermentation in batch cultures of these strains was optimal at about 35 ºC and an initial pH of 6.5, which for all strains gradually dropped to ca. pH 4 during the fermentation. Clostridium roseum H5 and C. diolis RT2 had the highest hydrogen yields per total substrate (120 ml H2/g initial COD). Substrate consumption alone by C. beijerinckii UAM and C. diolis RT2 reached 573 and 475 ml H2/g consumed COD, respectively. Butyric acid fermentation was predominant, with butyrate and acetate as the major by-products and propionate, ethanol, and lactate as secondary metabolites. The acetate butyrate ratios and fermentation pathways varied depending on the strains and environmental conditions. Hydrogenogenesis was studied in greater detail in C. saccharobutylicum H1. In butyric acid fermentation by this representative strain, acetoacetate was detected as an intermediate metabolite. Hydrogenogenesis was also analyzed in an enrichment culture, which behaved similarly to the axenic cultures (AU)
RESUMEN
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Collection:
National databases
/
Spain
Health context:
SDG3 - Target 3.9 Reduce the amount of deaths produced by dangerous chemicals and the pollution of the air, water and soil
Health problem:
Water, Sanitation and Hygiene
Database:
IBECS
Main subject:
Clostridium
/
Fermentation
/
Wastewater
/
Hydrogen
Language:
English
Journal:
Int. microbiol
Year:
2013
Document type:
Article
Institution/Affiliation country:
Autonomous University of Madrid/Spain
/
Mygen Laboratory/Spain