Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats
J. physiol. biochem
; 69(3): 527-537, sept. 2013.
Article
in English
| IBECS
| ID: ibc-121672
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Indomethacin (IND) is a non-steroid anti-inflammatory agent that is known to induce severe gastric mucosal lesions. In this study, we investigated the protective effect of selenium (SEL), grape seed extract (GSE), and both on IND-induced gastric mucosal ulcers in rats. SpragueDawley rats (200250 g) were given SEL, GSE, and both by oral gavage for 28 days, and then gastric ulcers were induced by oral administration of 25 mg/kg IND. Malondialdehyde (MDA), non-enzymatic (reduced glutathione, GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants, prostaglandin E2 (PGE2) in gastric mucosa, and serum tumor necrosis factor Alpha (TNF-Alpha) were measured. Moreover, gastric ulcer index and preventive index were determined. Indomethacin increased the gastric ulcer index, MDA, TNF-Alpha, and decreased PGE2 and non-enzymatic (GSH) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidants. Pretreatment with SEL, GSE, and both significantly decreased the gastric ulcer index, MDA, and TNF and increased antioxidants and PGE2. Histopathological observations confirm the gastric ulcer index and biochemical parameters. Selenium and GSE have a protective effect against IND-induced gastric ulcers through prevention of lipid peroxidation, increase of GSH, activation of radical scavenging enzymes, PGE2 generation, and anti-inflammatory activity. Co-administration of GSE and SEL is more effective than GSE or SEL alone
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Selenium
/
Stomach Ulcer
/
Grape Seed Extract
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
J. physiol. biochem
Year:
2013
Document type:
Article
Institution/Affiliation country:
King Khalid University/Saudi Arabia
/
Mansoura University/Egypt