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Oleic acid decreases the expression of a cholesterol transport-related protein (NPC1L1) by the induction of endoplasmic reticulum stress in CaCo-2 cells
Chen, Jiangyuan; Zhang , Ying; Yang, Pu; Zong, Yiqiang; Qu, Shen; Liu, Zhiguo; Li, Qi.
Affiliation
  • Chen, Jiangyuan; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Zhang , Ying; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Yang, Pu; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Zong, Yiqiang; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Qu, Shen; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Liu, Zhiguo; Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology. Wuhan. People’s Republic of China
  • Li, Qi; Wuhan Polytechnic University. School of Biology and Pharmaceutical Engineering. Hubei. People’s Republic of China
J. physiol. biochem ; 67(2): 153-163, jun. 2011.
Article in English | IBECS | ID: ibc-122615
Responsible library: ES1.1
Localization: BNCS
RESUMEN
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ABSTRACT
The reported data indicate that oleic acid (OA) decreases cholesterol absorption. To explore the underlying mechanisms, the effects of OA on the expression of cholesterol transport-related proteins (NPC1L1, ABCG5/8, ACAT2, MTP) and the unfolded protein response (UPR) pathway were studied in CaCo-2 enterocytes by incubating CaCo-2 cells with taurocholate micelles or taurocholate micelles containing different concentrations of OA (0.25–1.0 mM). We show that OA effectively induces XBP1 mRNA splicing, a key component of the UPR signaling, and the expression of BiP and mature ATF6 proteins in a concentration-dependent manner, leading to the induction of endoplasmic reticulum (ER) stress and activation of the UPR. Interestingly, OA decreases NPC1L1 expression in a dose-dependent manner while it has no effects on ABCG5 and MTP mRNA level or SREBP-2, ABCG8, and ACAT2 protein level. In CaCo- (..) (AU)
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Collection: National databases / Spain Database: IBECS Main subject: Oleic Acids / Caco-2 Cells / Cholesterol Ester Transfer Proteins / Endoplasmic Reticulum Stress Limits: Humans Language: English Journal: J. physiol. biochem Year: 2011 Document type: Article Institution/Affiliation country: Huazhong University of Science and Technology/People’s Republic of China / Wuhan Polytechnic University/People’s Republic of China
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Collection: National databases / Spain Database: IBECS Main subject: Oleic Acids / Caco-2 Cells / Cholesterol Ester Transfer Proteins / Endoplasmic Reticulum Stress Limits: Humans Language: English Journal: J. physiol. biochem Year: 2011 Document type: Article Institution/Affiliation country: Huazhong University of Science and Technology/People’s Republic of China / Wuhan Polytechnic University/People’s Republic of China