Identification of valid reference housekeeping genes for gene expression analysis in tumor neovascularization studies
Clin. transl. oncol. (Print)
; 15(3): 211-218, mar. 2013. tab, ilus
Article
in English
| IBECS
| ID: ibc-127080
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
INTRODUCTION:
Real time RT-PCR is a widely used technique to evaluate and confirm gene expression data obtained in different cell systems and experimental conditions. However, there are many conflicting reports about the same gene or sets of gene expression. A common method is to report the interest gene expression relative to an internal control, usually a housekeeping gene (HKG), which should be constant in cells independently of experimental conditions. MATERIALS ANDMETHODS:
In this study, the expression stability of ten HKGs was considered in parallel in two cell systems (endothelial and osteosarcoma cells) beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box binding protein (TBP), hypoxanthine phosphoribosyl-transferase 1 (HPRT1), Cyclophilin A (PPIA), beta-2-microglobulin (B2M), glucuronidase beta (GUSB), eukaryotic translation elongation factor 1 alpha1 (EEF1A1), transferrin receptor (TFRC), ribosomal protein S18 (RPS18). In order to study the stability of candidate reference genes, data have been also analyzed by several algorithms (geNorm, NormFinder, BestKeeper and delta-Ct method). RESULTS ANDCONCLUSIONS:
The overall analysis obtained by the comprehensive ranking showed that RPS18 and PPIA are appropriate internal reference genes for tumor neovascularization studies where it is necessary to analyze both systems at the same time (AU)
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Osteoblasts
/
Bone Neoplasms
/
Endothelium, Vascular
/
Osteosarcoma
/
Genes, Essential
/
Neovascularization, Pathologic
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Clin. transl. oncol. (Print)
Year:
2013
Document type:
Article
Institution/Affiliation country:
IRCCS SDN Foundation/Italy
/
Second University of Naples/Italy