A multiplex PCR for the simultaneous detection of Tenacibaculum maritimum and Edwardsiella tarda in aquaculture
Int. microbiol
; 17(2): 111-117, jun. 2014. ilus, tab
Article
in En
| IBECS
| ID: ibc-127305
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
A specific and sensitive multiplex PCR (mPCR) method was developed as a useful tool for the simultaneous detection of two important flatfish pathogens in marine aquaculture, Tenacibaculum maritimum and Edwardsiella tarda. In fish tissues, the average detection limit for these mPCR-amplified organisms was 2 × 105 ± 0.2 CFU/g and 4 × 105 ± 0.3 CFU/g, respectively. These values are similar or even lower than those previously obtained using the corresponding single PCR. Moreover, mPCR did not produce any nonspecific amplification products when tested against 36 taxonomically related and unrelated strains belonging to 33 different bacterial species. Large amounts of DNA from one of the target bacterial species in the presence of low amounts from the other did not have a significant effect on the amplification sensitivity of the latter (AU)
RESUMEN
No disponible
Full text:
1
Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Water Microbiology
/
Aquaculture
/
Edwardsiella tarda
/
Tenacibaculum
Type of study:
Diagnostic_studies
Language:
En
Journal:
Int. microbiol
Year:
2014
Document type:
Article