Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma
Med. oral patol. oral cir. bucal (Internet)
; 19(6): e562-e568, nov. 2014. ilus, tab
Article
in English
| IBECS
| ID: ibc-130349
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell prolifera tion and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of FADD and FAS genes in risk of OSCC. MATERIAL ANDMETHODS:
we analyze the promoter methylation status of FADD and FAS genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also, FADD and FAS genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse- Transcripts PCR.RESULTS:
Aberrant promoter methylation of FADD and FAS genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both FADD and FAS genes (P<0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both FADD (p < 0.02) and FAS (p < 0.007) genes.CONCLUSIONS:
To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the FADD and FAS genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations
Full text:
Available
Collection:
National databases
/
Spain
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Mouth and Oropharynx Cancers
Database:
IBECS
Main subject:
Carcinoma, Squamous Cell
/
Gene Expression
/
DNA Methylation
Limits:
Humans
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2014
Document type:
Article
Institution/Affiliation country:
Center Zahedan University of Medical Sciences/Iran
/
University of Sistan and Baluchestan/Iran
/
Zahedan University of Medical Sciences/Iran