Molecular genetic study of novel biomarkers for early diagnosis of oral squamous cell carcinoma
Med. oral patol. oral cir. bucal (Internet)
; 20(2): e167-e179, mar. 2015. ilus, tab
Article
in English
| IBECS
| ID: ibc-134129
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
OBJECTIVES:
Early detection and treatment of an oral squamous cell carcinoma (OSCC) is critical because of its rapid growth, frequent lymph-node metastasis, and poor prognosis. However, no clinically-valuable methods of early diagnosis exist, and genetic analysis of OSCCs has yielded no biomarkers. StudyDESIGN:
We investigated the expression of genes associated with inflammation in OSCCs via a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of microarray data. Tumor and normal tissues from five patients with an OSCC were used for microarray analysis. Differentially-expressed genes, identified using permutation, local pooled error (LPE), t-tests, and significance analysis of microarrays (SAM), were selected as candidate genetic markers.RESULTS:
Two groups corresponding to tissue identity were evident, implying that their differentially-expressed genes represented biological differences between tissues. Fifteen genes were identified using the Student's pairedt-test (p < 0.05) and the SAM, with a false discovery rate of less than 0.02. Based on gene expression, these 15genes can be used to classify an OSCC. A genetic analysis of functional networks and ontologies, validated by using a qRT-PCR analysis of the tissue samples, identified four genes, ADAM15, CDC7, IL12RB2 and TNFRSF8,that demonstrated excellent concordance with the microarray data.CONCLUSIONS:
Our study demonstrated that four genes (ADAM15, CDC7, IL12RB2 and TNFRSF8) had potential as novel biomarkers for the diagnosis and the treatment of an OSCC
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Odontogenic Tumor, Squamous
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2015
Document type:
Article
Institution/Affiliation country:
Ajou University School of Medicine/Republic of Korea
/
Korea University Guro Hospital/Republic of Korea
/
Seoul National University/Republic of Korea