Modulation of intestinal L-glutamate transport by luminal leptin
J. physiol. biochem
; 71(2): 311-317, jun. 2015.
Article
in English
| IBECS
| ID: ibc-140538
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Leptin is secreted into the digestive tract and contributes to the absorption of dietary molecules by regulating transporters activity. Here, we studied the effect of luminal leptin on the intestinal transport of L-glutamate, an important component of human diet. We examined the effect of leptin on L-glutamate uptake in rat intestine in vitro measuring glutamate-induced short-circuit current (Isc) in Ussing chambers and L-[3H (U)]-glutamate uptake in jejunal everted rings. Glutamate-induced Isc was only observed in Na+-free conditions. This Isc was concentration (160 mmol L−1) and pH dependent. Luminal leptin increased glutamate Isc (∼100 %). Dose-response curve showed a biphasic pattern, with maximal stimulations observed at 10−13 and 10−10 mmol L−1, that were sensitive to leptin receptor antagonist. In everted rings, two glutamate transport mechanisms were distinguished a Na+-dependent, H+-independent, that was inhibited by leptin (∼20 %), and a Na+-independent but H+-dependent, that was enhanced by leptin (∼20 %), in line with data obtained in Ussing chambers. Altogether, these data reveal original non-monotonic effect of luminal leptin in the intestine and demonstrate a new role for this hormone in the modulation of L-glutamate transport, showing that luminal active gut peptides can influence absorption of amino acids
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Leptin
/
Vesicular Glutamate Transport Proteins
Limits:
Animals
Language:
English
Journal:
J. physiol. biochem
Year:
2015
Document type:
Article
Institution/Affiliation country:
Faculté de Médicine Xavier Bichat/France
/
University of Navarra/Spain