Solar water disinfection (SODIS): impact on hepatitis A virus and on a human Norovirus surrogate under natural solar conditions
Int. microbiol
; 18(1): 41-49, mar. 2015. ilus, tab
Article
in English
| IBECS
| ID: ibc-141143
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
This study evaluates the effectiveness of solar water disinfection (SODIS) in the reduction and inactivation of hepatitis A virus (HAV) and of the human Norovirus surrogate, murine Norovirus (MNV-1), under natural solar conditions. Experiments were performed in 330 ml polyethylene terephthalate (PET) bottles containing HAV or MNV-1 contaminated waters (103 PFU/ml) that were exposed to natural sunlight for 2 to 8 h. Parallel experiments under controlled temperature and/or in darkness conditions were also included. Samples were concentrated by electropositive charged filters and analysed by RT-real time PCR (RT-qPCR) and infectivity assays. Temperature reached in bottles throughout the exposure period ranged from 22 to 40ºC. After 8 h of solar exposure (cumulative UV dose of ~828 kJ/m2 and UV irradiance of ~20 kJ/l), the results showed significant (P < 0.05) reductions from 4.0 (±0.56) ×104 to 3.15 (±0.69) × 103 RNA copies/100 ml (92.1%, 1.1 log) for HAV and from 5.91 (±0.59) × 104 to 9.24 (±3.91) × 103 RNA copies/100 ml (84.4%, 0.81 log) for MNV-1. SODIS conditions induced a loss of infectivity between 33.4% and 83.4% after 4 to 8 h in HAV trials, and between 33.4% and 66.7% after 6 h to 8 h in MNV-1 trials. The results obtained indicated a greater importance of sunlight radiation over the temperature as the main factor for viral reduction (AU)
RESUMEN
No disponible
Full text:
Available
Collection:
National databases
/
Spain
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
IBECS
Main subject:
Drinking Water
/
24961
/
Water Disinfection
/
Hepatitis A virus
/
Norovirus
Language:
English
Journal:
Int. microbiol
Year:
2015
Document type:
Article
Institution/Affiliation country:
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)/Spain
/
University of Santiago de Compostela/Spain