Exhaled breath condensate annexin A5 levels in exercise-induced bronchoconstriction in asthma: A preliminary study

ABSTRACT

BACKGROUND AND OBJECTIVE: The pathogenesis of exercise-induced bronchoconstriction (EIB) in asthma is incompletely understood. The role of exhaled breath condensate (EBC) annexin A5, which is an anti-inflammatory mediator, has not been investigated. The purpose of this study is to evaluate EBC annexin A5 levels in EIB in asthmatic children. METHODS: Two groups of children were enrolled in this study asthmatic children with positive (n = 11) and negative (n = 7) responses to exercise. The levels of pre- and post-exercise EBC annexin A5 were determined with using enzyme-linked immunosorbent assay (ELISA). RESULTS: We observed significant higher pre-exercise EBC annexin A5 levels in the challenge test negative children than in the challenge test positive children (p < 0.05). No significant difference was observed in the post-exercise EBC annexin A5 levels between the groups (p > 0.05). Also, no significant difference was observed between pre- and post-exercise EBC annexin A5 levels within each group (p > 0.05). There was an inverse correlation between annexin A5 levels and a reduction in forced expiratory volume at one second percent (FEV1%) (p = 0.009, r = −0.598). CONCLUSIONS: Our preliminary study showed that EBC annexin A5 may have a possible preventive role in EIB in asthma. Annexin A5 and related compounds may provide novel therapeutic approaches to the treatment of EIB in asthma

RESUMEN

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