Induction of nasal polyps using house dust mite and Staphylococcalenterotoxin B in C57BL/6 mice
Allergol. immunopatol
; 44(1): 66-75, ene.-feb. 2016. ilus, graf
Article
in English
| IBECS
| ID: ibc-147486
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
The murine polyp model was developed previously using ovalbumin and Staphylococcus aureusenterotoxin B (SEB). Here, we established a model mimicking key aspects of chronic eosinophilic rhinosinusitis with nasal polyps using the house dust mite (HDM), a clinically relevant aeroallergen, co-administered with SEB. We assessed the inflammatory response and formation of nasal polypoid lesions in an experimental murine model using intranasal delivery of HDM and ovalbumin.METHODS:
After induction of HDM-induced allergic rhinosinusitis in C57BL/6 mice, SEB (10 ng) was instilled into the nasal cavity of mice for eight weeks. Phosphate-buffered saline-challenged mice served as control. Histopathological changes were evaluated using haematoxylin and eosin for overall inflammation, Sirius red for eosinophils, and periodic acid-Schiff stain for goblet cells. The distribution of mast cells in mouse nasal tissue was determined by immunohistochemistry. Serum total IgE was measured using enzyme-linked immunosorbent assay.RESULTS:
Compared to mice treated with HDM only, the HDM + SEB-treated mice demonstrated nasal polypoid lesion formation and a significant increase in the number of secretory cells and eosinophilic infiltration. Moreover, mice challenged intranasally with HDM showed highly abundant mast cells in the nasal mucosa. In contrast, OVA + SEB-challenged mice showed a significantly lower degree of mast cell infiltration.CONCLUSION:
We established an in vivo model of chronic allergic rhinosinusitis with nasal polypoid lesions using HDM aeroallergen. This study demonstrated that the HDM + SEB-induced murine polyp model could be utilised as a suitable model for nasal polyps, especially with both eosinophil and mast cell infiltrationRESUMEN
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Sinusitis
/
Nasal Polyps
/
Models, Animal
/
Eosinophils
/
Hypersensitivity, Immediate
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Allergol. immunopatol
Year:
2016
Document type:
Article
Institution/Affiliation country:
Boramae Medical Center/Republic of Korea
/
Seoul National University College of Medicine/Republic of Korea