Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques
Med. oral patol. oral cir. bucal (Internet)
; 21(3): e276-e284, mayo 2016. tab
Article
in English
| IBECS
| ID: ibc-152707
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
The prevalence and amounts of periodontal pathogens detected in bacteraemia samples after tooth brushing-induced by means of four diagnostic technique, three based on culture and one in a molecular-based technique, have been compared in this study. MATERIAL ANDMETHODS:
Blood samples were collected from thirty-six subjects with different periodontal status (17 were healthy, 10 with gingivitis and 9 with periodontitis) at baseline and 2 minutes after tooth brushing. Each sample was analyzed by three culture-based methods [direct anaerobic culturing (DAC), hemo-culture (BACTEC), and lysis-centrifugation (LC)] and one molecular-based technique [quantitative polymerase chain reaction (qPCR)]. With culture any bacterial isolate was detected and quantified, while with qPCR only Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were detected and quantified. Descriptive analyses, ANOVA and Chi-squared tests, were performed.RESULTS:
Neither BACTEC nor qPCR detected any type of bacteria in the blood samples. Only LC (2.7%) and DAC (8.3%) detected bacteraemia, although not in the same patients. Fusobacterium nucleatum was the most frequently detected bacterial species.CONCLUSIONS:
The disparity in the results when the same samples were analyzed with four different microbiological detection methods highlights the need for a proper validation of the methodology to detect periodontal pathogens in bacteraemia samples, mainly when the presence of periodontal pathogens in blood samples after tooth brushing was very seldom
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Periodontitis
/
Bacteremia
/
Dental Plaque
Type of study:
Diagnostic study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2016
Document type:
Article
Institution/Affiliation country:
Santiago de Compostela University/Spain
/
University Complutense/Spain
/
Xeral-Cíes Hospital/Spain