Emergencia de Providencia rettgeri NDM-1 en dos departamentos de Colombia, 2012-2013 / Emergence of Providencia rettgeri NDM-1 in two departments of Colombia, 2012-2013
Enferm. infecc. microbiol. clín. (Ed. impr.)
; 35(6): 354-358, jun.-jul. 2017. tab, graf
Article
in Spanish
| IBECS
| ID: ibc-163475
Responsible library:
ES1.1
Localization: BNCS
RESUMEN
Introducción:
En Colombia, entre 2012 y 2013 se identificaron 19 aislamientos con NDM, de los cuales 14 correspondían a Providencia rettgeri.Métodos:
Los aislamientos se identificaron con Vitek-2, y la sensibilidad antimicrobiana se evaluó por microdilución en caldo. Las carbapenemasas se determinaron fenotípicamente con test de Hodge modificado y pruebas de sinergia con EDTA/SMA y APB, genotípicamente por PCR usando iniciadores específicos para KPC, GES, IMP, VIM, OXA-48 y NDM, y las relaciones genéticas se establecieron con Diversilab.Resultados:
Los aislamientos fueron resistentes a carbapenémicos, cefalosporinas de tercera generación, piperacilina-tazobactam, amicacina, gentamicina y tigeciclina, excepto a aztreonam. Todos los aislamientos fueron positivos para EDTA/SMA y NDM-1 y negativos para APB y otras carbapenemasas. Se definieron 2 grupos genéticos grupo 1 (n = 9 aislamientos), grupo 2 (n = 4 aislamientos), y un aislamiento no relacionado genéticamente.Conclusión:
Este trabajo describe la circulación de grupos de P. rettgeri productores de NDM-1 en Colombia (AU)ABSTRACT
Introduction:
In Colombia, between 2012 and 2013, 19 isolates with NDM were identified, of which 14 corresponded to Providencia rettgeri.Methods:
The isolates were identified by Vitek-2, and antimicrobial susceptibility was evaluated by broth microdilution. The carbapenemase phenotypes were determined with Modified Hodge Test and synergy tests with EDTA/SMA and APB, the genotypes by PCR using specific primers for KPC, GES, IMP, VIM, OXA-48 and NDM, and genetic relationships were established with DiversiLab.Results:
The isolates were resistant to carbapenems, third-generation cephalosporins, piperacillintazobactam, amikacin, gentamicin and tigecycline, except aztreonam. All isolates were positive for EDTA/SMA and NDM-1, and negative for APB and other carbapenemases. Two genetic groups, group 1 (n = 9 isolates), group 2 (n = 4 isolates) and an isolate defined as not genetically related.Conclusion:
This work describes the circulating of NDM-1-producing P. rettgeri in Colombia (AU)
Full text:
Available
Collection:
National databases
/
Spain
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
IBECS
Main subject:
Providencia
/
Beta-Lactams
/
Enterobacteriaceae
/
Enterobacteriaceae Infections
/
Klebsiella pneumoniae
Type of study:
Etiology study
/
Prognostic study
/
Risk factors
Limits:
Humans
Country/Region as subject:
South America
/
Colombia
Language:
Spanish
Journal:
Enferm. infecc. microbiol. clín. (Ed. impr.)
Year:
2017
Document type:
Article
Institution/Affiliation country:
Instituto Nacional de Salud/Colombia