Identification of lactobacilli residing in chicken ceca with antagonism against Campylobacter
Int. microbiol
; 14(2): 103-110, jun. 2011. tab, graf, ilus
Article
in English
| IBECS
| ID: ibc-164538
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Bacteriocins produced by Lactobacillus salivarius have been recently recognized as a natural means to control Campylobacter and Salmonella in live poultry. This finding is of relevance since Campylobacter jejuni and Campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. In the present work, lactic acid bacteria (LAB) isolated from the cecum of twenty Tunisian chickens were identified and those isolates with antagonism against Campylobacter were further characterized. Following their preliminary confirmation as LAB, 150 strains were identified by combining morphological criteria, biochemical tests, and molecular methods, the latter inluding intergenic 16S- 23S PCR, specific lactobacilli PCR, and a biphasic approach. Most of the LAB isolated belonged to the genus Lactobacillus, among them Lb. sakei (33.3%), Lb. salivarius (19.4%), Lb. reuteri (8.6%), and Lb. curvatus (8.6%). The other LAB strains included those of the genus Weissella (16.7%), Enterococcus faecalis (5.3%), Leuconostoc mesenteroides (2.7%), Lactococcus graviae (2.7%), and Streptococcus sp. (2.7%). The Lactobacilli strains were tested for their antagonism against C. jejuni and C. coli. The activity of three of them, Lb. salivarius SMXD51, Lb. salivarius MMS122, and Lb. salivarius MMS151, against the aforementioned target strains could be ascribed to the production of bacteriocins (AU)
RESUMEN
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Cecum
/
Campylobacter jejuni
/
Campylobacter coli
/
Lactobacillales
/
Antibiosis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Country/Region as subject:
Africa
Language:
English
Journal:
Int. microbiol
Year:
2011
Document type:
Article
Institution/Affiliation country:
Faculty of Sciences of Tunis/Tunisia
/
LUNAM University/France
/
University of Lille North of France/France