Sarcolipin expression is repressed by endoplasmic reticulum stress in C2C12 myotubes
J. physiol. biochem
; 73(4): 531-538, nov. 2017. graf, ilus
Article
in Spanish
| IBECS
| ID: ibc-178903
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Sarcolipin is a transmembrane protein expressed in the sarco/endoplasmic reticulum of skeletal and atrial muscles in large animals. Sarcolipin plays crucial roles in heat production through modifying the function of sarco/endoplasmic reticulum Ca2+ ATPase, thereby being involved in thermogenesis and systemic metabolism. In skeletal muscle, endoplasmic reticulum (ER) stress has been implicated in several conditions, such as insulin resistance, muscle diseases, and hypo/hyper-contraction. Here, we investigated the effect of ER stress on sarcolipin expression in skeletal muscle cells, C2C12 myotubes. First, gene expression of sarcolipin was confirmed in the cells during myogenesis. Then, ER stress was induced in C2C12 myotubes by treatment with tunicamycin or thapsigargin. Sarcolipin messenger RNA (mRNA) and protein expression were significantly reduced by ER stress induction. The reduction was independent of inositol-requiring element 1 (IRE1), which is activated by ER stress and has potent endonuclease activity, when evaluated by treatment with an IRE1 inhibitor, 4μ8C. On the other hand, sarcolipin mRNA stability was reduced under the ER stress when evaluated by treatment with actinomycin D. In conclusion, these results show that ER stress represses sarcolipin expression due to changes in mRNA stability in C2C12
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Proteolipids
/
Muscle Fibers, Skeletal
/
Endoplasmic Reticulum Stress
/
Muscle Proteins
Limits:
Animals
Language:
Spanish
Journal:
J. physiol. biochem
Year:
2017
Document type:
Article
Institution/Affiliation country:
Health Sciences University of Hokkaido Hospital/Japan
/
Health Sciences University of Hokkaido Hospital/Japana
/
Health Sciences University of Hokkaido/Japan
/
Hokkaido University/Japan
/
Sysmex Corporation/Japan