Hepatic subcellular distribution of squalene changes according to the experimental setting
J. physiol. biochem
; 74(4): 531-538, nov. 2018. ilus, graf
Article
in English
| IBECS
| ID: ibc-179031
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Squalene is the main unsaponifiable component of virgin olive oil, the main source of dietary fat in Mediterranean diet, traditionally associated with a less frequency of cardiovascular diseases. In this study, two experimental approaches were used. In the first, New Zealand rabbits fed for 4 weeks with a chow diet enriched in 1% sunflower oil for the control group, and in 1% of sunflower oil and 0.5% squalene for the squalene group. In the second, APOE KO mice received either Western diet or Western diet enriched in 0.5% squalene for 11 weeks. In both studies, liver samples were obtained and analyzed for their squalene content by gas chromatography-mass spectrometry. Hepatic distribution of squalene was also characterized in isolated subcellular organelles. Our results show that dietary squalene accumulates in the liver and a differential distribution according to studied model. In this regard, rabbits accumulated in cytoplasm within small size vesicles, whose size was not big enough to be considered lipid droplets, rough endoplasmic reticulum, and nuclear and plasma membranes. On the contrary, mice accumulated in large lipid droplets, and smooth reticulum fractions in addition to nuclear and plasma membranes. These results show that the squalene cellular localization may change according to experimental setting and be a starting point to characterize the mechanisms involved in the protective action of dietary squalene in several pathologies
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Cell Membrane
/
Diet, Mediterranean
/
Disease Models, Animal
/
Non-alcoholic Fatty Liver Disease
/
Liver
Limits:
Animals
Language:
English
Journal:
J. physiol. biochem
Year:
2018
Document type:
Article
Institution/Affiliation country:
CITA-Universidad de Zaragoza/Spain
/
Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza/Spain
/
Instituto de Salud Carlos IIIP1Center for Biomedical Research in the Network of the Pathophysiology of Obesity and Nutrition (CIBER)/Spain
/
Universidad de Zaragoza/Spain
/
University of Zaragoza/Spain