Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
Med. oral patol. oral cir. bucal (Internet)
; 24(5): e583-e587, sept. 2019. ilus, tab
Article
in English
| IBECS
| ID: ibc-185674
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Background:
The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out the presence of transmissible organic material after standard procedures. Material andMethods:
A total of 55 healing abutments previously used in patients will be washed and sterilized in a steam autoclave at 121ºC for 15 min. Each healing abutment will be cultured in Brain Heart Infusion broth (BHI) under strict aseptic conditions. Besides, two control groups will be included one of 3 unused healing abut-ments, and the other of just medium. After 10 days at 37°C under a 5% CO2 100 μl of the broth will be plated on solid media (Brain Infusion Agar, BHIA) and Columbia Blood agar to test for sterility. The remaining volume will be centrifuged, the sediment fixed, and a Gram stain performed to discard the presence of non-cultivable micro-organisms. Moreover, to determine the presence of remaining organic material after the cleaning and sterilizing treatments, the bioburden will be determined by measuring total organic carbon (TOC) in another 10 previously used healing abutments, cleaned and sterilized, that will be submerged in Milli-Q water and sonicatedRESUMEN
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Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Dental Implants
/
Dental Abutments
Limits:
Humans
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2019
Document type:
Article
Institution/Affiliation country:
University of Barcelona/Spain