Different methods of cell quantification can lead to different results: a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients
Med. oral patol. oral cir. bucal (Internet)
; 25(3): e431-e438, mayo 2020. ilus, tab, graf
Article
in English
| IBECS
| ID: ibc-196333
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
Although new digital pathology tools have improved the positive cell quantification, there is a heterogeneity of the quantification methods in the literature. The aim of this study was to evaluate and propose a novel dendritic cells quantification method in squamous cell carcinoma comparing it with a conventional quantification method. MATERIAL ANDMETHODS:
Twenty-six squamous cell carcinomas HIV-positive cases affecting the oropharynx, lips and oral cavity were selected. Immunohistochemistry for CD1a, CD83, and CD207 was performed. The immunohistochemical stains were evaluated by automated examination using a positive pixel count algorithm. A conventional quantification method (unspecific area method; UA) and a novel method (specific area method; SA) were performed obtaining the corresponding density of positive dendritic cells for the intratumoral and peritumoral regions. The Mann-Whitney U test was used to verify the influence of the quantification methods on the positive cell counting according to the evaluated regions. Data were subjected to the ANOVA and Student's t-test to verify the influence of the tumour location, stage, histological grade, and amount of inflammation on the dendritic cells density counting.RESULTS:
The cell quantification method affected the dendritic cells counting independently of the evaluated region (P-value < 0.05). Significant differences between methods were also observed according to the tumour features evaluations.CONCLUSIONS:
The positive cell quantification method influences the dendritic cells density results. Unlike the conventional method (UA method), the novel SA method avoids non-target areas included in the hotspots improving the reliability and reproducibility of the density cell quantificationRESUMEN
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Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Dendritic Cells
/
Mouth Neoplasms
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Carcinoma, Squamous Cell
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Oropharyngeal Neoplasms
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HIV Infections
Limits:
Adult
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Female
/
Humans
/
Male
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2020
Document type:
Article
Institution/Affiliation country:
Federal University of Santa Catarina/Brazil
/
Instituto do Câncer do Estado de São Paulo- Faculdade de Medicina da Universidade de São Paulo (ICESP-FMUSP)/Brazil
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Universidad de Talca/Chile
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University of Campinas (UNICAMP)/Brazil
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University of Pretoria/South Africa