Analysis of bacterial communities of infected primary teeth in a Mexican population
Med. oral patol. oral cir. bucal (Internet)
; 25(5): e668-e674, sept. 2020. tab, graf
Article
in English
| IBECS
| ID: ibc-196523
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
BACKGROUND:
The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing. MATERIAL ANDMETHODS:
Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed.RESULTS:
Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well.CONCLUSIONS:
The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalentRESUMEN
No disponible
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Bacteria
/
Tooth, Deciduous
/
Dental Pulp Cavity
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Country/Region as subject:
Mexico
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2020
Document type:
Article
Institution/Affiliation country:
División de Biología Molecular/México
/
División de Ciencias Ambientales/México
/
Universidad Autónoma de San Luis Potosí/México