MiR-181a promotes cell proliferation and migration through targeting KLF15 in papillary thyroid cancer
Clin. transl. oncol. (Print)
; 24(1): 66-75, enero 2022.
Article
in English
| IBECS
| ID: ibc-203415
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
IntroductionPapillary thyroid cancer (PTC) is the predominant histological type of thyroid cancer, accounting for 80% of thyroid cancers. MiR-181a is a novel microRNA that is usually upregulated in multiple cancers. This study aims to explore the role and underlying mechanism of miR-181a in PTC.MethodsCCK8 and Transwell assays were performed to evaluate cell viability and migration. The mRNA level of miR-181a and KLF15 was calculated by qRT-PCR. The protein level of E-Cadherin, N-Cadherin and GAPDH was evaluated by western blot. Dual luciferase assay was conducted to validate that miR-181a directly targeting the 3′-UTR of KLF15 mRNA in TPC-1 cells.ResultsWe observed that miR-181a was overexpressed and KLF15 was low expressed in PTC tissues and cell lines. Upregulation of miR-181a or downregulation of KLF15 predicted poor outcomes in PTC patients. MiR-181a improved cell growth of PTC, migration and epithelialmesenchymal transition (EMT) in TPC-1 cells. KLF15 was a target gene of miR-181a and its expression was mediated by miR-181a. KLF15 partially reversed the facilitating effect of miR-181a on cell proliferation and migration in TPC-1 cells.ConclusionWe discovered that miR-181a served as an oncogene downregulating KLF15, thereby inhibiting cell proliferation, migration and the EMT. These findings demonstrate that miR-181a plays a significant role in PTC progression and could be a therapeutic target for PTC.
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Thyroid Neoplasms
/
MicroRNAs
/
Health Sciences
/
Thyroid Cancer, Papillary
Limits:
Humans
Language:
English
Journal:
Clin. transl. oncol. (Print)
Year:
2022
Document type:
Article
Institution/Affiliation country:
Jinan Central Hospital/China
/
Qingdao Hospital of Traditional Chinese Medicine/China
/
Rizhao Hospital of TCM/China
/
Yantaishan Hospital/China
/
Zhangqiu District Peoples Hospital/China