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Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention
Liu, Shasha; Da, Jingjing; Yu, Jiali; Dong, Rong; Yuan, Jing; Yu, Fuxun; Zha, Yan.
Affiliation
  • Liu, Shasha; Guizhou University. School of Medicine. Guiyang. China
  • Da, Jingjing; Guizhou University. School of Medicine. Guizhou Provincial People's Hospital. Guiyang. China
  • Yu, Jiali; Guizhou University. School of Medicine. Guizhou Provincial People's Hospital. Guiyang. China
  • Dong, Rong; Guizhou Provincial People's Hospital. Department of Nephrology. NHC Key Laboratory of Pulmonary Immunological Disease. Guiyang. China
  • Yuan, Jing; Guizhou Provincial People's Hospital. Department of Nephrology. Guiyang. China
  • Yu, Fuxun; Guizhou Provincial People's Hospital. NHC Key Laboratory of Pulmonary Immunological Disease. Guiyang. China
  • Zha, Yan; Guizhou University. School of Medicine. Department of Nephrology, Guizhou Provincial People's Hospital. Guiyang. China
J. physiol. biochem ; 78(2): 389-399, May. 2022.
Article in English | IBECS | ID: ibc-215967
Responsible library: ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Diabetic kidney disease (DKD) is a major health burden closely related to lipid metabolism disorders. Leptin has lipid-lowering efficacy, but the specific mechanism of its local effects on kidney is still unclear. This study aims to investigate the role of ectopic lipid deposition (ELD) in DKD and evaluate the lipid-lowering efficacy of leptin in the palmitic acid (PA)-induced renal tubular epithelial cells (NRK-52E). DKD model was established in Sprague–Dawley (SD) rats by giving single intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat diet for 8 weeks. Then, the expression changes of lipid metabolism-related markers were observed. At week 12, the protein expression level of lipid-deposited marker adipose differentiation-related protein (ADRP) was significantly increased. Besides, the lipid synthesis marker sterol regulatory element-binding protein 1c (SREBP 1c) was highly expressed while the expression of insulin-induced gene 1 (Insig-1), a key molecular of inhibiting SREBP 1c, was decreased. Leptin and compound c were incubated with the PA-induced NRK-52E cells to investigate the lipid-lowering effects and whether this effect was mediated by the AMPK/Insig-1/SREBP 1c signaling pathways. mRNA and protein of ADRP and SREBP 1c were reduced after leptin treatment, while Insig-1 and phosphorylated AMP-activated protein kinase (AMPK) were increased. Conversely, inhibition of AMPK phosphorylation by compound c mostly eliminated lipid-lowering efficacy of leptin in PA-induced cells. Collectively, these results suggested that there was ELD of renal tubular epithelial cells in DKD rats. Leptin upregulated the expression level of Insig-1 by activating AMPK to attenuate ELD in PA-induced NRK-52E cells. (AU)
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Collection: National databases / Spain Database: IBECS Main subject: Diabetes Mellitus / Diabetic Nephropathies Limits: Animals Language: English Journal: J. physiol. biochem Year: 2022 Document type: Article Institution/Affiliation country: Guizhou Provincial People's Hospital/China / Guizhou University/China
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Collection: National databases / Spain Database: IBECS Main subject: Diabetes Mellitus / Diabetic Nephropathies Limits: Animals Language: English Journal: J. physiol. biochem Year: 2022 Document type: Article Institution/Affiliation country: Guizhou Provincial People's Hospital/China / Guizhou University/China
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