A pan-cancer analysis of RNASEH1, a potential regulator of the tumor microenvironment
Clin. transl. oncol. (Print)
; 25(8): 2569-2586, aug. 2023. ilus, graf
Article
in English
| IBECS
| ID: ibc-222432
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Background RNASEH1 (Ribonuclease H1) encodes an endonuclease that specifically degrades the RNA of RNADNA hybrids and acts in DNA replication and repair. Although there are many studies on RNASEH1, the research of RNASEH1 in cancers is still insufficient. Therefore, in order to clarify the physiological mechanism of RNASEH1 in tumor cells, we evaluated the role of RNASEH1 by combining The Cancer Genome Atlas (TCGA) pan-cancer data and Genotype-Tissue Expression (GTEx) normal tissue data. Methods RNASEH1 expression was analyzed by using RNAseq data from TCGA and the GTEx database. The Human Protein Atlas (HPA), GeneCards and STRING database were used to explore the protein information of RNASEH1. The prognostic value of RNASEH1 was analyzed by using the clinical survival data from TCGA. Differential analysis of RNASEH1 in different cancers was performed by using R package DESeq2, and enrichment analysis of RNASEH1 was conducted by using R package clusterProfiler. We downloaded the immune cell infiltration score of TCGA samples from published articles and online databases, and the correlation analysis between immune cell infiltration levels and RNASEH1 expression was performed. Not only that, we further evaluated the association of RNASEH1 with immune activating genes, immunosuppressive genes, chemokines and chemokine receptors. At the end of the article, the differential expression of RNASEH1 in pan-cancer was validated by using GSE54129, GSE40595, GSE90627, GSE106937, GSE145976 and GSE18672, and qRT-PCR was also performed for verification. Finding RNASEH1 was significantly overexpressed in 19 cancers and the overexpression was closely correlated with poor prognosis. Moreover, the expression of RNASEH1 was significantly correlated with the regulation of the tumor microenvironment (AU)
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Ribonuclease H
/
Tumor Microenvironment
/
Neoplasms
Limits:
Humans
Language:
English
Journal:
Clin. transl. oncol. (Print)
Year:
2023
Document type:
Article
Institution/Affiliation country:
Huazhong University of Science and Technology/China
/
Nanchang Hangkong University/China