Expression of caveolin-1 in tooth germ, ameloblastoma and ameloblastic carcinoma / Expresión de caveolina-1 en germen dental ameloblastoma y carcinoma ameloblástico
Med. oral patol. oral cir. bucal (Internet)
; 26(2): e238-e245, Mar. 2021. ilus, tab, graf
Article
in English
| IBECS
| ID: ibc-224444
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Background:
The caveolin-1 protein (structural component of membrane caveolae) plays important roles in sev-eral biological functions, such as endocytosis, cell adhesion, and cell signaling. However, this protein has been as-sociated with mechanisms of tumorigenesis in several neoplasms. The expression patterns and roles of caveolin-1in the oral epithelium and in embryonic and odontogenic tumor tissues are still unclear.Material andMethods:
The expression of caveolin-1 was evaluated in samples of the normal gingival epithelium(n=7), human tooth germ (TG) (n=12), ameloblastoma (AM) (n=83), and ameloblastic carcinoma (AC) (n=9) byimmunohistochemistry. Additionally, AM samples were analyzed by qRT-PCR and Western blot.Results:
Most TG (91.7%), AM (73.5%) and AC (100%) samples showed diverse patterns of immunohistochemicalpositivity for caveolin-1, while only one gingival sample was positive. The transcript levels of cav-1 were signifi-cantly upregulated by 14.9-fold in AM tissue (P = 0.0014) compared to those in normal gingival epithelial tissue,as shown by qRT-PCR. Presence of caveolin-1 protein was confirmed by Western blot analysis. The caveolin-1immunoexpression patterns throughout the stages of TG show its importance during odontogenesis.Conclusions:
The overexpression of caveolin-1 in AM and AC compared to its expression in normal gingivalepithelium (adult tissue) suggests a possible role of caveolin-1 in protumoral events, but due to the similar immu-noexpression observed in AM and AC, caveolin-1 may not necessarily participate in the malignant transformationprocess. However, future studies are needed to clarify and confirm these hypotheses.(AU)
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Immunohistochemistry
/
Ameloblastoma
/
Odontogenic Tumors
/
Oral Health
/
Caveolin 1
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Med. oral patol. oral cir. bucal (Internet)
Year:
2021
Document type:
Article
Institution/Affiliation country:
Universidad Autónoma Metropolitana/Mexico
/
Universidad Central de Venezuela/Venezuela
/
Universidad Juarez del Estado de Durango/Mexico
/
University of Parma/Italy
/
University of the Republic/Uruguay