Auraptene alleviates inflammatory injury and cell apoptosis in children with pneumonia in vitro
Allergol. immunopatol
; 51(6): 54-59, 2023. graf
Article
in English
| IBECS
| ID: ibc-227309
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Objective:
The aim of the present study is to investigate the effects of auraptene on inflammation and apoptosis of pneumonia cell model and uncover the mechanism.Methods:
WI-38 cells were treated with lipopolysaccharide (LPS) to construct a pneumonia model. Cell counting kit-8 assay, enzyme-linked-immunosorbent serologic assay, and quantitative polymerase chain reaction assay were conducted to confirm the effects of auraptene on the viability and inflammation of WI-38 cells. Flow cytometry (FCM) and immunoblot assays were conducted to detect the effects of auraptene on the apoptosis of WI-38 cells. Immunoblot assay was performed to confirm the mechanism.Results:
We found that auraptene stimulated cell viability in WI-38 cells upon LPS treatment. Auraptene also inhibited cellular inflammation. Furthermore, auraptene inhibited cell apoptosis of WI-38 cells upon LPS treatment. Mechanically, auraptene inhibited the nuclear factor kappa B signaling pathway, thereby suppressing the pneumonia.Conclusion:
Auraptene alleviates inflammatory injury and cell apoptosis in pneumonia, thus has the potential to act as a pneumonia drug (AU)
Full text:
Available
Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Pneumonia
/
Apoptosis
/
MicroRNAs
Limits:
Child
/
Humans
Language:
English
Journal:
Allergol. immunopatol
Year:
2023
Document type:
Article
Institution/Affiliation country:
Xiangnan University/China