Evaluation of the efficacy of hydroxyl radical (OH) release for disinfection of the air and surfaces in the dental clinic: an in vitro study / Evaluación de la eficacia de la liberación de radicales hidroxilo (OH) para la desinfección del aire y superficies en la clínica dental: un estudio in vitro

ABSTRACT

Background: Concerning about the quality of room air has increased exponentially. Specially in dental clinicswhere diary practice is characterized by the important generation of aerosols.Material and Methods: An in vitro model was used in which samples were collected from the surfaces and roomair of a dental clinic before and after the use of an OH˙ radical generator.Results: A total of 1260 samples were collected for bacteriological analysis and 14 samples for the detection ofSARS-CoV-2. Following OH˙ treatment, the tested surface samples showed a decrease in the number of colonyforming units (CFUs) of 76.9% in TSA culture medium. The circulating room air samples in turn showed adecrease in CFUs of 66.7% in Sabouraud medium and 71.4% in Mannitol agar medium. No presence of SARS-CoV-2 was observed on the surface of the face shield.Conclusions: The disinfectant technology based on the use of hydroxyl radicals (OH˙) is effective in reducing thepresence of moulds and yeasts and Staphylococcus in the air, and in reducing total aerobic bacteria on the testedsurfaces.(AU)