In vitro adhesion and anti-inflammatory properties of Limosilactobacillus fermentum FS-10 isolated from infant fecal sample / Adhesión in vitro y propiedades antiinflamatorias de Limosilactobacillus fermentum FS-10 aislado de muestra fecal infantil
Int. microbiol
; 27(1): 227-238, Feb. 2024. graf, tab
Article
in English
| IBECS
| ID: ibc-230256
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
In this study, seven strains of Limosilactobacillus fermentum were isolated from an infant fecal sample and characterized using in vitro studies. Lactobacillus rhamnosus GG was used as a comparison because it is a well-documented commercial probiotic. The isolates were tested for attributes such as acid and phenol tolerance, bile salt hydrolase (BSH) activity, and antibiotic sensitivity. One isolate, L. fermentum FS-10, displayed enhanced cell surface hydrophobicity (> 85%) and mucin adhesion. Mucin-binding helps colonization in the gut. The immunomodulatory property of L. fermentum FS-10 was evaluated by determining the modulation of pro- and anti-inflammatory factors such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-10, and nitric oxide (NO) in human acute monocytic leukemia (THP-1) cells under inflammatory conditions induced by lipopolysaccharide (LPS). L. fermentum FS-10 potently downregulated the expression of TNF-α and nitric oxide and upregulated IL-10 levels, indicating an anti-inflammatory response. Safety assessment of the strain revealed the absence of genes for virulence factors, toxin production, and antibiotic resistance, potentiating application as a probiotic strain.(AU)
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Tumor Necrosis Factor-alpha
/
Probiotics
/
Coliforms
/
Feces
/
Anti-Inflammatory Agents
Limits:
Child
/
Female
/
Humans
/
Male
Language:
English
Journal:
Int. microbiol
Year:
2024
Document type:
Article
Institution/Affiliation country:
Tata Chemicals Limited/India