Long Non-Coding RNA NUTM2A-AS1/miR-376a-3p/PRMT5 Axis Promotes Prostate Cancer Progression
Arch. esp. urol. (Ed. impr.)
; 77(2): 173-182, mar. 2024. ilus, graf, tab
Article
in English
| IBECS
| ID: ibc-231939
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Background:
In recent years, significant attention has been directed towards long non-coding RNA NUT family member 2A antisense RNA 1 (NUTM2A-AS1) for its oncogenic role in tumours. This study aimed to investigate the functional and molecular mechanisms underlying NUTM2A-AS1 in prostate cancer (PCa).Methods:
NUTM2A-AS1, miR-376a-3p, and protein arginine methyltransferase 5 (PRMT5) levels were assessed in PCa samples and matched non-cancerous prostate samples. The DU145 cell line was conditioned to undergo transfection with relevant plasmids, and a cell counting kit-8 assay was performed to evaluate cell proliferation. A Transwell assay was conducted to analyse cell migration or invasion. Cell apoptosis was assessed using an annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit and flow cytometry. A tumour sphere formation assay was conducted to assess the ability of PCa cells to form tumour spheres.Results:
We found elevated expression of NUTM2A-AS1 and PRMT5 and decreased expression of miR-376a-3p in PCa samples. Inhibition of NUTM2A-AS1 or overexpression of miR-376a-3p led to reduced cell proliferation and diminished cancer stem cell-like traits in vitro. NUTM2A-AS1 regulated miR-376a-3p through competitive absorption, thereby modulating PRMT5. Up-regulation of PRMT5 nullified the therapeutic effects of inhibiting NUTM2A-AS1 or overexpressing miR-376a-3p in DU145 cells.Conclusions:
NUTM2A-AS1 promotes cancer stem cell-like traits in PCa cells by targeting PRMT5 through miR-376a-3p. Therefore, these NUTM2A-AS1-based novel insights into tumour therapy hold promise for patients with PCa. (AU)
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Collection:
National databases
/
Spain
Database:
IBECS
Main subject:
Prostatic Neoplasms
/
Protein-Arginine N-Methyltransferases
/
MicroRNAs
/
RNA, Long Noncoding
Language:
English
Journal:
Arch. esp. urol. (Ed. impr.)
Year:
2024
Document type:
Article
Institution/Affiliation country:
Jinan Central Hospital/China
/
Qingdao West Coast New Area Peoples Hospital (Qingdao Huangdao District Peoples Hospital)/China
/
The Second Affiliated Hospital of Shandong First Medical University/China
/
Zibo Central Hospital/China