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Isolation and identification of bacteria from the invasive pest Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and evaluation of their biocontrol potential
Eski, Ardahan; Erdoğan, Pervin; Demirbağ, Zihni; Demir, İsmail.
Affiliation
  • Eski, Ardahan; Bilecik Seyh Edebali University. Vocational School. Program of Biomedical Equipment Technology. Bilecik. Turkey
  • Erdoğan, Pervin; Sivas University of Science and Technology. Faculty of Agricultural Sciences and Technology. Department of Plant Protection. Sivas. Turkey
  • Demirbağ, Zihni; Karadeniz Technical University. Faculty of Science. Department of Biology. Trabzon. Turkey
  • Demir, İsmail; Karadeniz Technical University. Faculty of Science. Department of Biology. Trabzon. Turkey
Int. microbiol ; 27(2): 631-643, Abr. 2024. graf
Article in English | IBECS | ID: ibc-232307
Responsible library: ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
As an alternative to chemical insecticides, gut bacteria of insects could be used to control insect pests. In this study, bacteria associated with Tuta absoluta, an invasive species that has developed resistance to chemical insecticides, were isolated, and their potential for pest control was investigated. We isolated 13 bacteria from larvae of the pest and identified the isolates on the basis of their morphological, physiological, biochemical, and molecular characteristics as Bacillus thuringiensis (Ta1-8), Staphylococcus petrasii (Ta9), Citrobacter freundii (Ta10), Chishuiella changwenlii (Ta11), Enterococcus casseliflavus (Ta12), and Pseudomonas tremae (Ta13). A laboratory screening test at 109 cfu/ml showed that B. thuringiensis (Bt) isolates caused more than 90% mortality after 3 days. Among the isolates, Bt-Ta1 showed the highest mortality in a short time. The LC50 and LC90 values for Bt-Ta1 were estimated to be 1.2 × 106 and 2 × 109 cfu/ml, respectively. Detailed characterization of Bt-Ta1 revealed that it is one of the serotypes effective on lepidopterans and contains the genes cry1Aa, cry2Aa, and vip3Aa, which encode lepidopteran toxic proteins. Bt-Ta1 isolate has been shown to have the potential to be used in the integrated management of Tuta absoluta. (AU)
Subject(s)
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Collection: National databases / Spain Database: IBECS Main subject: Bacteria / Agrochemicals / Agricultural Pests / Insecticides / Insecta Language: English Journal: Int. microbiol Year: 2024 Document type: Article Institution/Affiliation country: Bilecik Seyh Edebali University/Turkey / Karadeniz Technical University/Turkey / Sivas University of Science and Technology/Turkey
Search on Google
Collection: National databases / Spain Database: IBECS Main subject: Bacteria / Agrochemicals / Agricultural Pests / Insecticides / Insecta Language: English Journal: Int. microbiol Year: 2024 Document type: Article Institution/Affiliation country: Bilecik Seyh Edebali University/Turkey / Karadeniz Technical University/Turkey / Sivas University of Science and Technology/Turkey
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