Ascorbate-dependent decrease of the mucosal immune inflammatory response to gliadin in coeliac disease patients
Allergol. immunopatol
; 40(1): 3-8, ene.-feb. 2012.
Article
in English
| IBECS
| ID: ibc-96251
Responsible library:
ES1.1
Localization: BNCS
ABSTRACT
Background:
The IL-15/NF-KappaB axis has an important role in coeliac disease (CD) and may represent a molecular target for immunomodulation. Ascorbate (vitamin C) is known to show inhibitory effects on NF-KappaB. Therefore, we studied if ascorbate supplementation to gliadin gliadin-stimulated biopsy culture could down-regulate the mucosal immune response to gliadin in CD.Methods:
Duodenal biopsy explants from treated CD patients were gliadin challenged in vitro (100ìg/ml) with and without 20mM ascorbate. An extra tissue explant in basal culture was used as internal control. Secretion levels of nitrites (3h), and IFNGamma, TNFalpha, IFNalpha, IL-17, IL-13, and IL-6 (24h) were measured on the supernatants. IL-15 was assayed by western-blot on whole protein duodenal explants.Results:
The addition of ascorbate to in vitro culture gliadin-challenged biopsies blocked the secretion of nitrites (p=0.013), IFNGamma (p=0.0207), TNFalpha (p=0.0099), IFNá (p=0.0375), and IL-6 (p=0.0036) compared to samples from non-ascorbate supplemented culture. Cytokine secretion was downregulated by ascorbate even to lower values than those observed in basal cultures (IFNGamma p=0.0312; TNFalpha p=0.0312; IFNá p=0.0312; and IL-6 p=0.0078). Gliadin-challenge induced IL-15 production in biopsies from treated CD patients, while the addition of ascorbate to culture medium completely inhibited IL-15 production. Moreover, the inhibition of IL-15 by ascorbate took place even in the only treated CD-patient who had basal IL-15 production.Conclusions:
Ascorbate decreases the mucosal inflammatory response to gluten in an intestinal biopsy culture model, so it might have a role in future supplementary therapy in CD(AU)
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Collection:
National databases
/
Spain
Health context:
Sustainable Health Agenda for the Americas
Health problem:
Goal 9: Noncommunicable diseases and mental health
/
Goal 10: Communicable diseases
Database:
IBECS
Main subject:
Ascorbate Oxidase
/
Celiac Disease
/
Immunity, Mucosal
/
Gliadin
Limits:
Humans
Language:
English
Journal:
Allergol. immunopatol
Year:
2012
Document type:
Article
Institution/Affiliation country:
Hospital Clínico Universitario/Spain
/
Universidad de Valladolid-CSIC/Spain