Increased TNF-Alpha, IL-6 and decreased IL-1â immunohistochemical expression by the stromal spindle-shaped cells in the centralgiant cell granuloma of the jaws

ABSTRACT

Objectives: the exp ress ion of the osteoclastogenic cytokines TNF-Alpha, IL-6 and IL-1Beta were immunohistochemically evaluated in peripheral (PGCG) and central (CGCG) giant cell granulomas of the jaws in order to determine differences between these two lesions and between the two distinct tumor cell populations (multinucleated giant cells, MGCs and stromal spindle-shaped cells).Study Design: Paraffin-embedded tissue sections from 40 PGCG and 40 CGCG were immunohistochemically stained using antibodies against TNF-Alpha, IL-6 and IL-1Beta. The percentage of positively stained cells and the staining intensity were assessed to provide a combined immunoreactivity score value. Results: TNF-Alpha, IL-6 and IL-1Beta were expressed in all lesions. The CGCG compared to the PGCG showed significantly increased expression of TNF-Alpha and IL-6 and decreased expression of IL-1Beta by the spindle-shaped cells and increased express ion of IL-1Beta by the MGCs. The MGCs demonstrated in comparison to the stromal spindle shaped cells significantly increased expression of all three cytokines in both PGCG and CGCG. Conclusions: The proinflammatory cytokines TNF-Alpha, IL-6 and IL-1Beta seem to be involved in the growth process of PGCG and CGCG of the jaws. A possible alteration in the synthesis or/and activity of these cytokines by the stromal spindle cells in the CGCGs may enhance osteolysis through the stimulation of osteoclast progenitor cells, given the fact that the intraosseous lesions cause bone resorption (AU)