Activation of yeast trehalase by heat shock
Braz. j. med. biol. res
; 24(10): 977-84, 1991. ilus, tab
Article
in English
| LILACS
| ID: lil-102078
Responsible library:
BR1.1
RESUMO
1. Activation of Saccharomyces cerevisiae trehalase by heat shock was shown in all strains tested, including mutants in which the reponse to a glucose signal was absent. A low concentration of cAMP favored the response as seen in 2nd log cells or in ras2 and cyr1ts mutant strains. The heat shock effect upon trehalse activity was not observed under conditions of catabolite repession. 2 Neither hexokinase PII nor the heat shock protein hsp26 seemed to be involve in the axtivation of trehalase by heat shock. However, mutant strains deleted in the polyubiquitin gene showed only a 2-fold activation of the enzyme while in control strains a 5-to 7-fold irreversible activation was observed. 3. An alternative mechanism of trehalase activation by removal of an inhibitor through ligation with ubiquitin is discussed. Activation by cAMP-independent phosphorylation is also considered
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Collection:
International databases
Database:
LILACS
Main subject:
Saccharomyces cerevisiae
/
Trehalase
/
Heat-Shock Proteins
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1991
Document type:
Article