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Protein-DNA associations in a gender-specific gene of Schistosoma mansoni: characterization by UV cross-linking, DNase I footprinting and band shift assays
Mem. Inst. Oswaldo Cruz ; 87(supl.4): 67-70, 1992. ilus
Article in English | LILACS | ID: lil-125628
Responsible library: BR15.1
ABSTRACT
Protein extracts obtained from male and female shistosomes were incubated with a gender-specific gene, F-10, transcribed only in adult females and encoding a major egg-shell protein. The protein/DNA interaction was measured using the band shift, DNase-I-footprinting and UV cross-linking techniques. The results showed a clear band shift when a 302 bp restriction fragment containing the 3'end of the gene was incubated with either female or male proteins. This fragment also contained a putative steroid hormone regulatory element (HRE). In contrast, only the male proteins produced a shift with the 495 bp fragment corresponding to the middle region of the gene. DNase I footprinting showed that proteins from males and females interacted with the F-10 gene by binding to multiple adjacent sites along the DNA, thus generatingrelatively long protected fragments of approximately 100 bp. This result suggested that the adjacent binding of several moles of proteins occured at the 5'end of the gene. UV cross-linking between schistosome proteins and a 21 bp synthetic oligonucleotide the F-10 HRE, evidence proteins having MWS of 30,45 and 65 kDNA. These proteins are presumably involved in the regulation of transcription of the F-10 gene
Subject(s)
Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Schistosomiasis / Zoonoses Database: LILACS Main subject: Schistosoma mansoni / Sexual Maturation / Bacterial Proteins Type of study: Risk factors Aspects: Social determinants of health Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 1992 Document type: Article
Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Schistosomiasis / Zoonoses Database: LILACS Main subject: Schistosoma mansoni / Sexual Maturation / Bacterial Proteins Type of study: Risk factors Aspects: Social determinants of health Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 1992 Document type: Article
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