Expresión de una proteína relacionada al receptor de estrógeno (p29), en carcinoma primario de la mama: análisis metodológico, anatomoclínico y del contenido de ADN / Expression of a estrogen related protein (p29) in primary breast carcinoma: methodological, anatomoclinical and DNA contents analysis

RESUMO

The aim of this work was to assess the immunohistochemical detector of a estrogen receptor related protein (p29) in 48 histological samples of primary mammary carcinoma and its relationship to clinical, morphological and ADN content parameters. p29 protein was positive in 62.5 per cent of samples. Over 50 per cent of samples had moderate or intense immunohistochemical staining (staining index over 16) and 77 per cent has a heterogenous expression of p29 protein. Sevently six per cent of p29 positive and 53 per cent of p29 negative tumors had a proliferation fraction over 10 per cent (determined by the fraction with flux cytometry). No relationship between p29 expression and the analyzed anatomoclinical variables was found. These results highlight this immunohistochemical method as an alternative to more complex and difficult biochemical thechniques. On the other hand the good results obtained in formalin fixed tissues allow retrospective studies in mammary carcinoma samples